Tubulin as a molecular component of coated vesicles.
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Tubulin as a molecular component of coated vesicles
Two proteins of 53,000 and 56,000 mol wt have been found to be associated with coated vesicles (CV) purified from bovine brain and chicken liver. These proteins share molecular weights, isoelectric points, and antigenic determinants with alpha- and beta-tubulins purified from bovine brain. Based on SDS PAGE and electron microscopic analysis of controlled pore glass bead exclusion column fractio...
متن کاملPurification of Coated Vesicles
We have applied agarose gel electrophoresis as a novel step in the purification of clathrincoated vesicles . Preparations of coated vesicles obtained by sedimentation velocity and isopycnic centrifugation are resolved into two distinct fractions upon electrophoresis . The slower migrating fraction contains smooth vesicles, whereas the faster contains only coated vesicles and empty clathrin coat...
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Eps15, a phosphorylation substrate of the epidermal growth factor (EGF) receptor kinase, has been shown to bind to the alpha-subunit of the clathrin-associated protein complex AP-2. Here we report that in cells, virtually all Eps15 interacts with the cytosol and membrane-bound forms of AP-2. This association is not affected by the treatment of cells with EGF. Immunofluorescence microscopy revea...
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Approximately one third of a cell's proteins are destined to function outside the cell's boundaries or while embedded within cellular membranes. Ensuring these proteins reach their diverse final destinations with temporal and spatial accuracy is essential for cellular physiology. In eukaryotes, a set of interconnected organelles form the secretory pathway, which encompasses the terrain that the...
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0350 February 2005 | Volume 3 | Issue 2 | e80 | e90 Correction: Components of Coated Vesicles and Nuclear Pore Complexes Share a Common Molecular Architecture Damien Devos, Svetlana Dokudovskaya, Frank Alber, Rosemary Williams, Brian T. Chait, Andrej Sali, Michael P. Rout DOI: 10.1371/journal.pbio.0020380 In PLoS Biology, volume 2, issue 12. In Materials and Methods, the sentence “The magnetic ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.97.4.1191